Coding

Part:BBa_K3796202

Designed by: Yiwei Wang   Group: iGEM21_CAU_China   (2021-09-07)


Phosphoenolpyruvate carboxylase (ppc) from C.glutamicum ATCC 13032

This part encodes phosphoenolpyruvate carboxylase(EC 4.1.1.31;GenBank: BAB98978.1),which catalyzes PEP to form OAA,which is important for the carbon flux from TCA cycle to the synthesis of amino acids. In our project, we changed its RBS to a stronger one to enhance the TCA cycle.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2167
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2167
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal BglII site found at 1617
    Illegal BglII site found at 2081
    Illegal BamHI site found at 2179
    Illegal BamHI site found at 2194
    Illegal XhoI site found at 871
    Illegal XhoI site found at 1516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2167
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2167
    Illegal NgoMIV site found at 1627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2001
    Illegal BsaI.rc site found at 767
    Illegal SapI.rc site found at 259


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